
今天做了一天的MEP填料动态载量实验,可是还是出现了很多问题,100mM NaCl时流穿太快,10%DBC=12mg左右,250mM条件时,中途暂停时间太长,使得蛋白与填料的结合时间增长,因此动态载量偏大。
MEP(Mercapto-Ethyl-Pyridine) Hypercel is a high capacity, high selectivity sorbent specially designed forthe capture and purification of monoclonal and polyclonal antibodies.
没有评论:
发表评论